Washing Test in Visual Studio .NET

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Washing Test
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In the washing test,8 subjects are asked to wash their face using a specific foaming agent such as soap or detergent After washing, subjects are asked to evaluate, according to a point scale, individual sensations of tightness, burning, itching, and stinging The aim of this test is to identify a subpopulation of people with an increased tendency to produce a skin response1
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Evaluation of Itch Response
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An itch response can be experimentally induced by topical or intradermal injections of various substances such as vasoactive agents, mast cell degranulators, and proteolytic enzymes One of the most common procedures to induce itching is the intradermal injection of histamine (100 g in 1 mL of saline) in the subject s forearm Information is then obtained by the subject s selfassessment regarding the intensity of the itch using a predetermined grading scale By using a similar evaluation technique (application of topical 4% histamine in a group of healthy young females), Grove postulated that there is
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For this test, baseline skin parameters are evaluated Then, a subject s hands and forearms are soaked in a solution of anionic surfactants such as soap, 005% sodium laureth sulfate, or 035% paraffin sulfonate at 40 C for 20 minutes After soaking, hands and
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forearms are rinsed with tap water and pat dried Then, the procedure is repeated 2 more times, with a 2-hour period between each soaking for 2 consecutive days Skin parameters are evaluated 2 hours after the third and sixth soakings and 18 hours after the last soaking All skin parameters are performed after the subject has rested at least 30 minutes in a 21 1C environment1
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the blood flow detected by the laser Doppler velocimetry (LDV)
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This tool allows for the quantitative and objective visualization of sebum excretion The amount and distribution of sebum vary individually, of course People with excess sebum (ie, oily skin) are also more likely to develop acne and, therefore, are probably more sensitive to breakouts when using topical products This objective assessment of sebum excretion may be superior to the subjective impression of skin type Manufacturers market product lines and cosmetics for various skin types The subjective impression of skin type can lead an individual to select an unsuitable product, ie, one that produces discomfort after application9 The Sebumeter is a photometric device whereby a special plastic strip is placed on a patient s skin and then inserted into the instrument The plastic strip absorbs the lipids on the skin surface and becomes more transparent The device measures the transparency and therefore sebum based on the light transmission through the strip The Sebumeter (SM 815; CourageKhazaka, K ln, Germany) is commercially available on the market and has been utilized in several studies for the measurement of facial sebum SEBUTAPE METHOD This is a more accurate and faster technique using a polymer film to measure lipid absorption The polymer tape absorbs sebum and becomes transparent to light afterwards Sebutape can be analyzed in many ways, the easiest of which is the visual scoring of the tapes on a 1 to 5 scale (Fig 38-2)
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The Evaporimeter or Tewameter (Transepidermal Water Loss)
This instrument measures water loss from the skin indicating disruption of the stratum corneum (SC) (Fig 38-1) Of course, TEWL is an important indicator of SC functioning Diminished function of the SC disrupts the skin barrier resulting in increased TEWL Maibach et al showed over a decade ago that TEWL increases at sites of damage, especially in people with a positive dermatologic history as well as hand and facial dermatitis, contact dermatitis, metal dermatitis, and textile itching10 Most cutaneous diseases that are characterized by abnormalities in the normal terminal differentiation process of keratinocytes are often associated with high TEWL Also, high TEWL is correlated with easier, higher permeability by external irritants, hence TEWL implies elevated sensitivity The evaporimeter test therefore appears to offer high sensitivity but low specificity in diagnosing sensitive skin (For more information, see Ref 11)
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