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TABLE 45 List of Laboratory Solutions, Reagents, and Indicators (Continued )
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2,5-Dinitrophenol (indicator): dissolve 01 g in 20 mL alcohol, then dilute with water to 100 mL; pH range colorless 4 58 yellow 2,6-Dinitrophenol (indicator): dissolve 01 g in a few mL alcohol, then dilute with water to 100 mL; pH range colorless 24 40 yellow Esbach s reagent (estimation of proteins): dissolve 10 g of picric acid and 20 g of citric acid in water and dilute to one liter Eschka s mixture (sulfur in coal): mix 2 parts of porous calcined MgO with 1 part of anhydrous Na2CO3; not a solution but a dry mixture Ether, (C2H5)2O use commercial grade p-Ethoxychrysoidine, p-ethoxybenzeneazo-m-phenylenediamine (indicator): dissolve 01 g of the base in 100 mL 90% alcohol; or, 01 g of the hydrochloride salt in 100 mL water; pH range red 35 55 yellow Ethyl bis-(2,4-dinitrophenyl) acetate (indicator): the stock solution is prepared by saturating a solution containing equal volumes of alcohol and acetone with the indicator; pH range colorless 74 91 deep blue This compound is available commercially The preparation of this compound is described by Fehnel and Amstutz, Ind Eng Chem, Anal Ed 16, 53 (1944), and by von Richter, Ber 21, 2470 (1888), who recommended it for the titration of orange- and red-colored solutions or dark oils in which the end-point of phenolphthalein is not easily visible The indicator is an orange solid which after crystallization from benzene gives pale yellow crystals melting at 150 1535 C, uncorrected Fehling s solution (sugar detection and estimation): (a) Copper sulfate solution: dissolve 34639 g of CuSO4 5H2O in water and dilute to 500 mL (b) Alkaline tartrate solution: dissolve 173 g of rochelle salts (KNaC4H4O6 4H2O) and 125 g of KOH in water and dilute to 500 mL Equal volumes of the two solutions are mixed just prior to use The Methods of the Assoc of Of cial Agricultural Chemists give 50 g of NaOH in place of the 125 g KOH Ferric chloride, FeCl3 05N: 27 g per liter Ferric nitrate, Fe(NO3)3 9H2O 05N: 67 g per liter Ferrous ammonium sulfate, Mohr s salt, FeSO4 (NH4)2SO4 6H2O 05N: 196 g per liter Ferrous sulfate, FeSO4 7 H2O 05N: 80 g per liter; add a few drops of H2SO4 Folin s mixture (for uric acid): dissolve 500 g of ammonium sulfate, 5 g of uranium acetate, and 6 mL of glacial acetic acid, in 650 mL of water The volume is about a liter Formal or Formalin: use the commercial 40% solution of formaldehyde Froehde s reagent (gives characteristic colorations with certain alkaloids and glycosides): dissolve 001 g of sodium molybdate in 1 mL of concentrated H2SO4; use only a freshly prepared solution Gallein (indicator): dissolve 01 g in 100 mL alcohol; pH range light brown-yellow 38 66 rose Glyoxylic acid solution (protein detection): cover 10 g of magnesium powder with water and add slowly 250 mL of a saturated oxalic acid solution, keeping the mixture cool; lter off the magnesium oxalate, acidify the ltrate with acetic acid and make up to a liter with water Guaiacum tincture: dissolve 1 g of guaiacum in 100 mL of alcohol Gunzberg s reagent (detection of HCl in gastric juice): dissolve 4 g of phloroglucinol and 2 g of vanillin in 100 mL of absolute alcohol; use only a freshly prepared solution
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APPLIED CHEMISTRY
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TABLE 45 List of Laboratory Solutions, Reagents, and Indicators (Continued )
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Hager s reagent (for alkaloids): this reagent is a saturated solution of picric acid in water Hanus solution (for determination of iodine number): dissolve 132 g of iodine in a liter of glacial acetic acid that will not reduce chromic acid; add suf cient bromine to double the halogen content determined by titration (3 mL is about the right amount) The iodine may be dissolved with the aid of heat, but the solution must be cold when the bromine is added Hematoxylin (indicator): dissolve 05 g in 100 mL alcohol; pH range yellow 50 60 Heptamethoxy red, 2,4,6,2 ,4 ,2 ,4 -heptamethoxytriphenyl carbinol (indicator): dissolve 01 g in 100 mL alcohol; pH range red 50 70 colorless Hydriodic acid, HI 05N: 64 g per liter Hydrobromic acid, HBr 05N: 40 g per liter Hydrochloric acid, HCl 5N: 182 g per liter; sp gr 1084 Hydro uoric acid, H2F2 48% solution: use as purchased, and keep in the special container Hydrogen peroxide, H2O2 3% solution: use as purchased Hydrogen sul de, H2S: prepare a saturated aqueous solution Indicator solutions: a number of indicator solutions are listed in this section under the names of the indicators; eg, alizarin, aurin, azolitmin, et al, which follow alphabetically See also various index entries Indigo carmine, sodium indigodisulfonate (indicator): dissolve 025 g in 100 mL 50% alcohol; pH range blue 116 140 yellow Indo-oxine, 5,8-quinolinequinone-8-hydroxy-5-quinoyl-5-imide (indicator): dissolve 005 g in 100 mL alcohol; pH range red 60 80 blue Cf Berg and Becker, Z Anal Chem 119, 81(1940) Iodeosin, tetraiodo uorescein (indicator): dissolve 01 g in 100 mL ether saturated with water; pH range yellow 0 about 4 rose-red; see also under methyl orange Iodic acid, HIO3 05N (HIO3 / 12): 15 g per liter Iodine: see tincture of iodine Lacmoid (indicator): dissolve 05 g in 100 mL alcohol; pH range red 44 62 blue Litmus (indicator): powder the litmus and make up a 2% solution in water by boiling for 5 minutes; pH range red 45 83 blue Lead acetate, Pb(C2H3O2)2 3H2O 05N: 95 g per liter Lead chloride, PbCl2 saturated solution is 1 / 7N Lead nitrate, Pb(NO3)2 05N: 83 g per liter Lime water: see calcium hydroxide Magnesia mixture: 100 g of MgSO4, 200 g of NH4Cl, 400 mL of NH4OH, 800 mL of water; each cc 001 g phosphorus P) Magnesium chloride, MgCl2 6H2O 05N: 50 g per liter Magnesium nitrate, Mg(NO3)2 6H2O 05N: 64 g per liter Magnesium sulfate, epsom salts, MgSO4 7H2O 05N: 62 g per liter; saturated solution dissolve 600 g of the salt in water and dilute to one liter Manganous chloride, MnCl2 4H2O 05N: 50 g per liter Manganous nitrate, Mn(NO3)2 6H2O 05N: 72 g per liter Manganous sulfate, MnSO4 7H2O 05N: 69 g per liter
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