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TABLE 45 List of Laboratory Solutions, Reagents, and Indicators (Continued )
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Marme s reagent (gives yellowish-white precipitate with salts of alkaloids): saturate a boiling solution of 4 parts of KI in 12 parts of water with CdI2; then add an equal volume of cold saturated KI solution Marquis reagent (gives a purple-red coloration, then violet, then blue with morphine, codeine, dionine, and heroine): mix 3 mL of concentrated H2SO4 with 3 drops of a 35% formaldehyde solution Mayer s reagent (gives white precipitate with most alkaloids in a slightly acid solution): dissolve 1355 g of HgCl2 and 50 g of KI in a liter of water Mercuric chloride, HgCl2 05N: 68 g per liter Mercuric nitrate, Hg(NO3)2 05N: 81 g per liter Mercuric sulfate, HgSO4 05N: 74 g per liter Mercurous nitrate, HgNO3: mix 1 part of HgNO3, 20 parts of H2O, and 1 part of HNO3 Metacresol purple, m-cresol-sulfonphthalein (indicator): dissolve 01 g in 136 mL 002N NaOH and dilute with water to 250 mL; acid pH range red 05 25 yellow, alkaline pH range yellow 74 90 purple Metanil yellow, diphenylaminoazo-m-benzene sulfonic acid (indicator): dissolve 025 g in 100 mL alcohol; pH range red 12 23 yellow Methyl green, hexamethylpararosaniline hydroxymethylate (component of mixed indicator): dissolve 01 g in 100 mL alcohol; when used with equal part of hexamethoxytriphenyl carbinol gives color change from violet to green at a titration exponent (pI) of 40 Methyl orange, orange III, tropeolin D, sodium p-dimethylaminoazobenzenesulfonate (indicator): dissolve 01 g in 100 mL water; pH range red 30 44 orange-yellow If during a titration where methyl yellow is being used a precipitate forms which tends to remove the indicator from the aqueous phase, methyl orange will be found to be a more suitable indicator This occurs, for example, in titrations of soaps with acids The fatty acids, liberated by the titration, extract the methyl yellow so that the end-point cannot be perceived Likewise methyl orange is more suitable for titrations in the presence of immiscible organic solvents such as carbon tetrachloride or ether used in the extraction of alkaloids for analysis Iodeosin (q v) has also been proposed as an indicator for such cases Cf Mylius and Foerster, Ber 24, 1482 (1891); Z Anal Chem 31, 240 (1892) Methyl red, p-dimethylaminoazobenzene-o -carboxylic acid (indicator): dissolve 01 g in 186 mL 002 N NaOH and dilute with water to 250 mL; or, 01 g in 60% alcohol; pH range red 44 62 yellow Methyl violet (indicator): dissolve 025 g in 100 mL water; pH range blue 15 32 violet Methyl yellow, p-dimethylaminoazobenzene, benzeneazodimethylaniline (indicator): dissolve 01 g in 200 mL alcohol; pH range red 29 40 yellow The color change from yellow to orange can be perceived somewhat more sharply than the change of methyl orange from orange to rose, so that methyl yellow seems to deserve preference in many cases See also under methyl orange Methylene blue, N,N,N ,N -tetramethylthionine (component of mixed indicator): dissolve 01 g in 100 mL alcohol; when used with equal part of methyl yellow gives color change from blue-violet to green at a titration exponent (pI) of 325; when used with equal part of 02% methyl red in alcohol gives color change from red-violet to green at a titration exponent (pI) of 54; when used with an equal part of neutral red gives color change from violet-blue to green at a titration exponent (pI) of 70 Millon s reagent (gives a red precipitate with certain proteins and with various phenols): dissolve 1 part of mercury in 1 part of HNO3 (sp gr 140) with gentle heating, then add 2 parts of water; a few crystals of KNO3 help to maintain the strength of the reagent
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