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General Biology
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The two major cell types are prokaryotic and eukaryotic The organelles found in eukaryotic cells are the nucleus, smooth ER, rough ER, Golgi complex, lysosomes, peroxisomes, and mitochondria Cell walls, chloroplasts, and the central vacuole are unique to plant cells The cell membrane is a phospholipid bilayer which is selectively permeable Substances can move across the membrane via passive, active, or bulk transport Enzymes speed chemical reactions by lowering activation energy Adenosine triphosphate (ATP) is the preferred energy source for all organisms Photosynthesis provides glucose and oxygen gas for other organisms The lightdependent reactions convert solar energy to chemical energy, producing oxygen as a byproduct, while the light-independent reactions convert carbon dioxide to glucose Cellular respiration is used to generate ATP as glucose is broken down Aerobic cellular respiration breaks down glucose to CO2 It involves glycolysis, the Krebs cycle, and the electron transport chain Oxygen serves as the terminal electron acceptor and ultimately binds to protons to produce water The net yield of ATP from aerobic cellular respiration far exceeds that from anaerobic respiration DNA is structured as a double helix Semiconservative replication is used to synthesize complementary strands of DNA The central dogma of molecular biology dictates the ow of genetic material in the cell: DNA RNA protein Protein synthesis is a two-step process Transcription converts DNA to mRNA Translation converts mRNA to a protein Mendelian laws assume that one allele is dominant, asserting itself over a recessive allele Punnett squares predict offspring given the genotypes of parents Monohybrid crosses consider one trait, while dihybrid crosses consider two traits together Mendel s theories do not consider certain genetic situations, such as incomplete dominance, codominance, polygenic inheritance, pleiotropy, epistasis, or environmental in uences Cell division occurs in two forms: mitosis and meiosis Mitosis is used for growth, repair, and replacement of cells Meiosis is used for sexual reproduction to produce gametes Evolution means change Individuals do not evolve, populations do Natural selection is the primary mechanism for evolution The Hardy-Weinberg equation is used to calculate allelic frequencies population The two equations are: p + q = 1 and p2 + 2pq + q2 = 1 Evidence for evolution occurs in the form of the fossil record, biogeography, comparative anatomy, and comparative embryology The origin of life is explained by several key theories including the big bang theory, endosymbiotic theory, and membrane infolding theory
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PART I BACTERIA PROKARYOTES
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There are three domains in which living organisms are classi ed: Eukarya, Bacteria, and Archaea All eukaryotic organisms are classi ed in the domain Eukarya, leaving all prokaryotic cells to be classi ed as either Bacteria or Archaea While both domains share the characteristics of being single celled, absorbing their nutrients, having a single loop of DNA, and lacking organelles, there are some differences between the two groups While Archaea used to be mistakenly classi ed as bacteria, their molecular and cellular structures were found to be quite different They have a unique cell wall, ribosomes, and membrane lipids Archaea live in very diverse environments and some species are termed extremophiles due to their habitats such as polar ice caps, thermal vents, extreme salt concentrations, jet fuel, and others Most Archaea species are anaerobes and none are known to be pathogens Because of their medical and environmental signi cance, the focus of this chapter will be on bacteria
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CLASSIFICATION OF BACTERIA
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Bacteria are extremely diverse The classi cation of bacteria is generally based on the mechanisms used by the particular species to obtain nutrients from the environment The following are the basic bacterial classi cations: Photoautotrophs: There are some bacterial species that produce their own nutrients through the process of photosynthesis, using carbon dioxide from the environment Photoheterotrophs: Bacteria that perform photosynthesis but cannot use carbon dioxide from the environment are considered photoheterotrophs In order to get the carbon needed for photosynthesis (which normally comes from carbon dioxide), they extract carbon from a variety of other sources Chemoautotrophs: These species get their energy from inorganic compounds, and their carbon needs are obtained from carbon dioxide Chemoheterotrophs: Energy is obtained for these species from inorganic substances while carbon is obtained from a variety of sources other than carbon dioxide These species are further subdivided based on the source of carbon they use Some species can extract carbon through parasitic or symbiotic interactions with a host or through the decomposition of other organisms Bacteria can also be classi ed based on their oxygen requirements, or lack thereof, for cellular respiration Some bacteria are obligate aerobes, always requiring oxygen for aerobic cellular respiration Other bacteria are obligate anaerobes, never needing oxygen, generally not dividing, and in some cases being killed by exposure to oxygen Finally, some bacteria are considered facultative anaerobes, requiring oxygen at times and not at other times
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