barcode scanning in c#.net PROBLEM 3: What role does the RecBCD protein play in in Software

Encoding QR Code 2d barcode in Software PROBLEM 3: What role does the RecBCD protein play in

PROBLEM 3: What role does the RecBCD protein play in
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mutants of Drosophila melanogaster. The ies differ in wing vein pattern. Are the mutations that cause these variants allelic Answer: To verify allelism, the investigator must create a heterozygote of the two mutations by either mating the ies, if they are of opposite sexes, or breeding each mutant into a separate stock for new matings. If the heterozygotes are of the wild-type, then the mutations are not allelic. If the heterozygote has a mutant phenotype, then we presume the mutations are functional alleles. (Allelism should be veri ed in females to be sure that the locus is not on the X chromosome, since males have only one.) If the mutations are functional alleles, it is possible to determine whether they are also structural alleles by looking for wildtype offspring of the heterozygote. If they occur at a rate higher than the background mutation rate, the alleles are not structural alleles. If wild-type offspring occur at the mutation rate, the alleles are presumably structural.
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PROBLEM 2: What is the difference between mismatch
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recombination Answer: For recombination to take place in E. coli, a single strand of DNA from the exogenote must insinuate itself into the chromosomal double helix with the help of the RecA protein. It is the RecBCD protein that creates the single-stranded DNA. It does so by traveling down the double helix, creating a single-strand tail in its wake. At chi sites, it switches the activity of the enzyme from a 3 5 exonuclease to a 5 3 exonuclease, creating a 3 tail. RecA can then act on this single-stranded tail to initiate recombination.
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1. Construct a data set that Luria and Delbr ck might have obtained that would prove the mutation theory wrong. 2. What types of enzymatic functions are best studied using temperature-sensitive mutations 3. Seven arginine-requiring mutants of E. coli were independently isolated. All pairwise matings were done (by transduction) to determine the number of loci (complementation groups) involved. If a ( ) in the following gure indicates growth and a ( ) no growth on minimal medium, how many complementation groups are involved Why is only half a table given Must the upper left to lower right diagonal be all ( )
*Answers to selected exercises and problems are on page A-13.
4. Several rII mutations (M to S) have been localized to the A cistron because of their failure to complement with a known deletion in that cistron. The phages carrying these mutations are then mated pairwise with the following series of subregion deletions.The mating is done on E. coli B and plated out on E. coli
Tamarin: Principles of Genetics, Seventh Edition
III. Molecular Genetics
12. DNA: Its Mutation, Repair, and Recombination
The McGraw Hill Companies, 2001
Exercises and Problems
K12 (see the gure). A ( ) shows the presence of plaques on K12, whereas a ( ) shows an absence of growth. Look at the deletion map of the area and localize each of the rII A mutations on this map.
Deletion Mutant M N O P Q R S 1 + + + + + 2 + + + 3 + + + 4 + + + + +
13. 5-bromouracil, 2-aminopurine, pro avin, ethyl ethane sulfonate, and nitrous acid are chemical mutagens. What does each do 14. A point mutation occurs in a particular gene. Describe the types of mutational events that can restore a functional protein, including intergenic events. Consider missense, nonsense, and frameshift mutations. 15. Why does misalignment result in addition or deletion of bases 16. What are the differences and similarities between intergenic and intragenic suppression 17. Eight independent mutants of E. coli requiring tryptophan (trp) are isolated. Complementation tests are performed on all pairwise combinations. Based on the results shown, determine how many genes you have identi ed and which mutants are in which genes ( complementation; no complementation).
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5. A Drosophila worker isolates four eye-color forms of the y: wild-type, white, carmine, and ruby. (The worker does not know that white, carmine, and ruby are three separate loci on the X chromosome.) What crosses could the researcher make to determine allelic relations of the genes What results would be expected A new mutant, eosin, is isolated. What crosses should be carried out to determine that eosin is an allele of white 6. De ne structural and functional alleles. What is the cis part of a cis-trans complementation test 7. Did Benzer and Yanofsky work with genes that had intervening sequences What relevance might introns have to their work 8. How can intra-allelic complementation result in incorrect conclusions about allelism 9. E. coli bacteria of strain K12 are lysogenic for phage . Why won t rII mutants of phage T4 grow in these bacteria 10. Diagram the tautomeric base pairings in DNA. What base pair replacements occur because of the shifts 11. What is the difference between a substrate and a template transition mutation 12. Describe two mechanisms for transversion mutagenesis.
18. Complementation tests are usually done with recessive mutations for if the mutations were dominant, all progeny would be mutant, regardless of whether the genes are allelic. Suppose you have isolated in a diploid species two independent dominant mutations that each confer resistance to the drug cycloheximide. Call these mutations Chx-1 and Chx-2. What crosses can you perform to determine whether the mutations are allelic Your crosses should allow you to determine whether the mutations are allelic, nonallelic and unlinked, or nonallelic and linked. 19. A series of overlapping deletions in phage T4 are isolated. All pairwise crosses are performed, and the progeny scored for wild-type recombinants. In the following table, wild-type progeny recovered; no wild-type progeny recovered.
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