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Tamarin: Principles of Genetics, Seventh Edition
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III. Molecular Genetics
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15. The Eukaryotic Chromosome
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The McGraw Hill Companies, 2001
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6. What is the protein composition of a nucleosome What function does histone H1 have 7. What are the relationships among the 110 , 300 , and 2,400 bers of the eukaryotic chromosome 8. Draw a mitotic chromosome during metaphase. Diagram the various kinds of bands that can be brought out by various staining techniques. What information is known about the DNA content of these bands 9. Give a 300 ber model of the chromosome to account for G-bands. 10. Give a 300 ber model of the chromosome to account for polytene chromosomal puffs. 11. What are the differences among polytene chromosomes, lampbrush chromosomes, puffs, and Balbiani rings Draw an example of each. 12. Under what circumstances does a chromosomal puff occur What does it signify 13. What is satellite DNA What does it signify 14. What is a centromere a kinetochore What do we know about the sequences within a yeast centromere 15. What is a telomere What are its functions What is its structure 16. Describe three ways in which cells protect their telomeres. 17. What functions exist in unique, repetitive, and highly repetitive DNAs 18. How would you use recombinant DNA techniques to locate the number and position of Alu members in the human chromosomes 19. How could you use modern recombinant DNA technology to determine the direction of transcription of the histone genes in gure 15.33 20. How many functional globin genes are there in mammals 21. How could you determine, using modern recombinant DNA techniques, that the - and -globin pseudogenes exist
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22. Kavenoff and colleagues determined the size of DNA in Drosophila chromosomes in two ways: (1) Spectrophotometric measurements were made on the largest intact chromosome. These measurements were then used to calculate the amount of DNA in each chromosome. (2) Nuclei were gently lysed and chromosomes isolated. The lengths of the longest DNA molecules were measured, and those lengths were used to determine the amount of DNA in each molecule. What results for each method would you expect if a. the chromosomes contain one DNA molecule b. the chromosomes contain more than one DNA molecule 23. What can be said about the base composition of the satellite DNA with a density of 1.671 in gure 15.21 24. When chromatin is partially digested with an endonuclease, the proteins removed, and the DNA separated in a sizing gel, DNA fragments in multiples of two hundred base pairs are found. Provide an explanation for this observation. 25. If chromatin is digested with an endonuclease to produce two hundred base-pair fragments, and these fragments are then used for transcription experiments, very little RNA is made. Provide an explanation for this observation. 26. Can nucleosomes contain the DNA for one gene Explain. 27. If radioactive probes are made from highly repetitive DNA, these probes hybridize in situ mainly to centromeric and telomeric regions. What does this result suggest about the organization of chromosomes 28. Would you expect archaeal species to have nucleosomes 29. What is the C-value paradox, and how is it explained 30. What are the origins of SINEs and LINEs
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1. How could comparative DNA studies aid us in understanding the roles of the different kinds of DNA present in the eukaryotic chromosome
2. How could mutations involving telomeres lead to cancer
Suggested Readings for chapter 15 are on page B-14.
Tamarin: Principles of Genetics, Seventh Edition
III. Molecular Genetics
16. Gene Expression: Control in Eukaryotes
The McGraw Hill Companies, 2001
GENE EXPRESSION
Control in Eukaryotes
STUDY OBJECTIVES
1. To examine the control of transcription in eukaryotes 2. To analyze the genetic control of development in eukaryotes 469 3. To study the mechanisms causing cancer 484 4. To study the genetic mechanisms that generate antibody diversity 492 465
STUDY OUTLINE
Control of Transcription in Eukaryotes 465 Chromatin Remodeling 465 Speci c Transcription Factors 466 Methylation of DNA 466 Signal Transduction 467 Transposons 468 Patterns in Development 469 Drosophila Development 469 Developmental Genetics of Drosophila 471 Plants 479 Other Models of Development 483 Cancer 484 Mutational Nature of Cancer 484 Viral Nature of Cancer 487 Environmental Causes of Cancer 492 Immunogenetics 492 Immunoglobulins 493 Antibody Diversity 494 T-Cell Receptors and MHC Proteins 498 Summary 505 Solved Problems 505 Exercises and Problems 506 Critical Thinking Questions 507 Box 16.1 Protein Motifs of DNA Recognition 480 Box 16.2 Chromosomal Painting 486 Box 16.3 AIDS and Retroviruses 502
Arti cially colored scanning electron micrograph of T-lymphocytes, white blood cells involved in the immune system. The cells are seen in the thymus gland, where they mature.
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