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III. Molecular Genetics
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17. Non Mendelian Inheritance
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4. Snail coiling is called a maternal trait. Is it possible that it is caused by an allele at a sex-linked locus 5. How would you rule out a viral origin for snail-shell coiling 6. Give the genotypes involved when a sinistral female snail produces dextral offspring. What genotypes could the male parent of the sinistral female have 7. A dextral snail is self-fertilized and produces only sinistral progeny. What is the probable genotype of this snail and its parents 8. In corn, male sterility is controlled by a maternal cytoplasmic element. A dominant nuclear gene, Restorer (Rf), restores fertility to male sterile lines. If pollen from a homozygous RfRf plant is used to pollinate a male sterile plant, what genotypes and phenotypes would you expect in the progeny
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9. What evidence indicates that it is not absolutely essential, in an evolutionary sense, for mitochondria to have genes for speci c components of oxidative phosphorylation 10. How would you determine that a segregative petite mutant in yeast is controlled by a chromosomal gene 11. What results would you obtain by making all possible pairwise crosses of the three types of yeast petites 12. An ornamental spider plant has green and white striped leaves. How can you determine whether cytoplasmic inheritance is responsible for the striping and whether there is interaction with an iojaptype chromosomal gene 13. In Chlamydomonas, 0.02% of the meiotic products are of the mt parental type. How can you use this information in mapping (Use streptomycin sensitivity, strs, and resistance, strr, as an example.) 14. What similarities do mitochondria and plastids share 15. What evidence is there that mitochondria and chloroplasts originated from prokaryotes 16. Individuals from killer and nonkiller strains of Paramecium are mixed together. Cytoplasmic exchange occurs during conjugation. Approximately 25% of the exconjugants are sensitive, and the remaining 75% are killers. What are the genotypes of the individuals of the two strains, and what ratios of sensitives and killers would result if the various exconjugants underwent autogamy 17. What genetic tests could you conduct to show that the mate-killer phenotype in Paramecium requires a dominant allele at any one of two loci
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18. Resistant and sensitive strains of Drosophila melanogaster differ in their ability to tolerate CO2 anesthetization with it kills sensitive ies. What genetic experiments would you perform to determine whether the trait is caused by a virus How would you rule out chromosomal genes 19. Suppose you have identi ed a person who has introns in his or her mitochondrial DNA. What would you deduce about the origin of this DNA 20. A mutation in the mitochondrial genome in people causes blindness. If reciprocal matings between affected and normal individuals occur in a family pedigree, what types of children would you expect from each cross 21. When chloroplast DNA from Chlamydomonas is digested with a particular restriction enzyme and then hybridized with a particular probe, two bands are detected. Some strains (type 1) yield bands of 1.5 and 3.7 kilobases; other strains (type 2) yield bands of 2.5 and 6.0 kilobases. For the following crosses, predict the progeny: a. mt , strain 1 mt , strain 2 b. mt , strain 2 mt , strain 1 22. What type of asci do you expect if you cross a yeast strain carrying an antibiotic resistance gene in its mitochondria with a strain that has normal (sensitive) mitochondria 23. In Paramecium, the maintenance of kappa particles requires the dominant nuclear gene K. A Kk killer cell conjugates with a sensitive cell of the same genotype without cytoplasmic exchange. Predict the genotypes and phenotypes that result if each exconjugant then undergoes autogamy. 24. In Neurospora, the slow-growing trait poky is inherited maternally and is due to an abnormal respiratory protein. A nuclear gene F makes poky individuals grow faster, even though the protein is still defective. Such strains are called fast-poky (F is normal poky). Poky cytoplasm is not altered by F in a zygote, and F has no effect on normal cytoplasm. What genotypes and phenotypes do you expect if the maternal parent is fast-poky and the paternal parent is normal 25. In corn, two independent, recessive nuclear genes, japonica (j) and iojap (ij), produce variegation (green and white striped leaves). Matings between individuals heterozygous for japonica always produce 3 green:1 striped individuals regardless of how the cross is performed. The behavior of iojap was described in gure 17.9. You have a variegated plant that could be either jj or ijij. What cross can you make to determine the genotype of this plant, and what results do you expect in the F1 generation in each case
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