barcode scanner code in c#.net Minimal Synthetic Medium for Growing in Software

Print QR Code ISO/IEC18004 in Software Minimal Synthetic Medium for Growing

Table 7.1 Minimal Synthetic Medium for Growing
Decoding QR Code In None
Using Barcode Control SDK for Software Control to generate, create, read, scan barcode image in Software applications.
Generate QR Code JIS X 0510 In None
Using Barcode creator for Software Control to generate, create QR Code 2d barcode image in Software applications.
E. coli, a Heterotroph
QR Code 2d Barcode Reader In None
Using Barcode scanner for Software Control to read, scan read, scan image in Software applications.
QR Generator In Visual C#
Using Barcode encoder for .NET Control to generate, create QR Code image in .NET applications.
Component NH4H2PO4 Glucose NaCl MgSO4 7H2O K2HPO4 H2O Quantity 1g 5g 5g 0.2 g 1g 1,000 ml
Denso QR Bar Code Maker In .NET
Using Barcode creation for ASP.NET Control to generate, create Quick Response Code image in ASP.NET applications.
QR Code Drawer In .NET Framework
Using Barcode maker for Visual Studio .NET Control to generate, create QR-Code image in .NET framework applications.
Figure 7.2 Con uent growth of bacterial colonies on a petri
Denso QR Bar Code Printer In Visual Basic .NET
Using Barcode drawer for .NET framework Control to generate, create QR Code JIS X 0510 image in .NET framework applications.
UPCA Generator In None
Using Barcode drawer for Software Control to generate, create UPC-A Supplement 5 image in Software applications.
Source: Data from M. Rogosa, et al., Journal of Bacteriology, 54:13, 1947.
Code 128 Code Set C Maker In None
Using Barcode printer for Software Control to generate, create Code 128 Code Set C image in Software applications.
Painting GTIN - 128 In None
Using Barcode printer for Software Control to generate, create UCC.EAN - 128 image in Software applications.
plate. Bacteria were streaked on the petri plate with an inoculation loop a metal wire with a looped end covered with bacteria. Streaks began at the upper right and continued around clockwise. With a heavy inoculation on the loop, bacterial growth is con uent. Eventually, only a few bacteria are left; they form single colonies at the upper left. (Photo by Robert Tamarin.)
Code 39 Full ASCII Creation In None
Using Barcode maker for Software Control to generate, create Code-39 image in Software applications.
Barcode Generator In None
Using Barcode generation for Software Control to generate, create bar code image in Software applications.
Tamarin: Principles of Genetics, Seventh Edition
Creating Code 2 Of 7 In None
Using Barcode drawer for Software Control to generate, create ANSI/AIM Codabar image in Software applications.
Data Matrix Creation In Java
Using Barcode generation for BIRT reports Control to generate, create Data Matrix ECC200 image in BIRT applications.
II. Mendelism and the Chromosomal Theory
Create Code-128 In None
Using Barcode creation for Word Control to generate, create Code 128 image in Microsoft Word applications.
UPC-A Drawer In None
Using Barcode maker for Font Control to generate, create Universal Product Code version A image in Font applications.
7. Linkage and Mapping in Prokaryotes and Bacterial Viruses
Make Barcode In Java
Using Barcode creator for Android Control to generate, create bar code image in Android applications.
UPCA Encoder In None
Using Barcode creation for Online Control to generate, create UCC - 12 image in Online applications.
The McGraw Hill Companies, 2001
GTIN - 12 Drawer In Java
Using Barcode generation for Android Control to generate, create UPC Symbol image in Android applications.
European Article Number 13 Scanner In None
Using Barcode scanner for Software Control to read, scan read, scan image in Software applications.
Bacterial Phenotypes
ent morphologies observed among the colonies are usually under genetic control ( g. 7.4).
Nutritional Requirements
The second basis for classifying bacteria by their nutritional requirements re ects the failure of one or more enzymes in the bacteria s biosynthetic pathways. If an auxotroph has a requirement for the amino acid cysteine that the parent strain (prototroph) does not have, then that auxotroph most likely has a nonfunctional enzyme in the pathway for the synthesis of cysteine. Figure 7.5 shows ve steps in cysteine synthesis; a different enzyme controls each step. All enzymes are proteins, and the information in one or more genes determines the sequences in the strings of amino acids that make up those proteins (chapter 11). A normal or wild-type allele
Viral plaques (phage T1) on a bacterial lawn of
E. coli.
( Bruce Iverson, BSc.)
The experimental cultivation of viruses is somewhat different. Since viruses are obligate parasites, they can grow only in living cells. Thus, for the cultivation of phages, petri plates of appropriate media are inoculated with enough bacteria to form a continuous cover, or bacterial lawn. This bacterial culture serves as a medium for the growth of viruses added to the plate. Since the virus attack usually results in rupture, or lysis, of the bacterial cell, addition of the virus usually produces clear spots, known as plaques, on the petri plates ( g. 7.3). Large quantities of viruses can be grown in asks of bacteria.
BACTERIAL PHENOTYPES
Bacterial phenotypes fall into three general classes: colony morphology, nutritional requirements, and drug or infection resistance.
Colony Morphology
The rst of these classes, colony morphology, relates simply to the form, color, and size of the colony that grows from a single cell. A bacterial cell growing on a petri plate in an incubator at 37 C divides as frequently as once every twenty minutes. Each cell gives rise to a colony, or clone, at its original position. In a relatively short amount of time (e.g., overnight), the colonies will consist of enough cells to be seen with the unaided eye. The differ(d) Figure 7.4
Various bacterial colony forms on agar petri plates. (a) Red and white colonies of Serratia marcescens. (b) Irregular raised folds of Streptomyces griseus. (c) Round colonies with concentrated centers and diffuse edges of Mycoplasma. (d) Irregularly folded raised colonies of Streptomyces antibioticus.
([a] Dr. E. Buttone/Peter Arnold, Inc., [b] C. Case/Visuals Unlimited, [c] Michael G. Gabridge/Visuals Unlimited, [d] Cabisco/Visuals Unlimited.)
Tamarin: Principles of Genetics, Seventh Edition
II. Mendelism and the Chromosomal Theory
7. Linkage and Mapping in Prokaryotes and Bacterial Viruses
The McGraw Hill Companies, 2001
Seven
Linkage and Mapping in Prokaryotes and Bacterial Viruses
Adenine H O H O O C Methionine adenosyltransferase CH NH2 Methionine CH2 CH2 S CH3 ATP + H2O Pi + PPi H O O C CH2 CH NH2 CH2 CH2 S+ CH3 H
OH OH
S-Adenosylmethionine
Adenine H O Methyltransferase H O O C CH2 CH NH2 S-Adenosylhomocysteine CH2 CH2 S H
OH OH
Methyl-group acceptor
Methylated acceptor
H Adenosylhomocysteinase O O C H2O Adenosine
CH NH2
Homocysteine
H Cystathionine -synthase O O C Serine H 2O
NH2 CH NH2 L-Cystathionine CH2 CH2 S CH2 C H COOH
Cystathionine -Lyase SH H2O NH3 -Ketobutyrate Cysteine Figure 7.5 CH2
NH2 C H COOH
Five-step conversion of methionine to cysteine. Each step is controlled by a different enzyme (red).
Tamarin: Principles of Genetics, Seventh Edition
II. Mendelism and the Chromosomal Theory
7. Linkage and Mapping in Prokaryotes and Bacterial Viruses
The McGraw Hill Companies, 2001
Bacterial Phenotypes
Sterile velvet No colony Incubate
Complete medium
Medium without methionine
Joshua Lederberg (1925 ).
(Courtesy of Dr. Joshua Lederberg.)
produces a normal, functional enzyme.The alternative allele may produce a nonfunctional enzyme. Recall the one-gene-one-enzyme hypothesis from chapter 2. A technique known as replica-plating, devised by Joshua Lederberg, is a rapid screening technique that makes it possible to determine quickly whether a given strain of bacteria is auxotrophic for a particular metabolite. In this technique, a petri plate of complete medium is inoculated with bacteria. The resulting growth will have a certain con guration of colonies. This plate of colonies is pressed onto a piece of sterilized velvet. Then any number of petri plates, each containing a medium that lacks some speci c metabolite, can be pressed onto this velvet to pick up inocula in the same pattern as the growth on the original plate ( g. 7.6). If a colony grows on the complete medium but does not grow on a plate with a medium missing a metabolite, the inference is that the colony is made of auxotrophic cells that require the absent metabolite. Samples of this bacterial strain can be obtained from the colony growing on complete medium for further study.The nutritional requirement of this strain is its phenotype. The methionine-requiring auxotroph of gure 7.6 would be designated as Met (methionine-minus or Met-minus). In terms of energy sources, the plus or minus notation has a different meaning. For example, a strain of bacteria that can utilize the sugar galactose as an energy source
Replica-plating technique. (a) A pattern of colonies from a plate of complete medium is transferred (b) to a second plate of medium that lacks methionine. (c) In the locations where colonies fail to grow on the second plate, we can infer that the original colony was a methionine-requiring auxotroph.
would be Gal . If it could not utilize galactose, it would be called Gal . The latter strain will not grow if galactose is its sole carbon source. It will grow if a sugar other than galactose is present. Note that a Met strain needs methionine to grow, whereas a Gal strain needs a carbon source other than galactose; it cannot use galactose.
Copyright © OnBarcode.com . All rights reserved.