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a. Devise a data set with no crossover interference that would yield these map units. b. What data would yield the same map units but with a coef cient of coincidence of 0.60 8. Ancon (an) and spiny legs (sple), from problem 7, are 10 map units apart on chromosome 3. Notchy (ny, wing tips nicked) is on the X chromosome (chromosome 1). Create a data set that would result if you were making crosses to determine the linkage arrangement of these three loci. How would you know that the notchy locus is on the X chromosome 9. In the house mouse, the autosomal alleles Trembling and Rex (short hair) are dominant to not trembling (normal) and long hair, respectively. Heterozygous Trembling, Rex females were crossed with normal, long-haired males and yielded the following offspring:
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Trembling, Rex Trembling, long-haired normal, Rex normal, long-haired 42 105 109 44
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kidney, cardinal ebony kidney, ebony cardinal kidney ebony, cardinal kidney, ebony, cardinal wild-type
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a. Determine the chromosomal composition of the F1 females. b. Derive a map of the three genes. 12. Following is a partial map of the third chromosome in Drosophila. 19.2 javelin bristles ( jv) 43.2 thread arista (th) 66.2 Delta veins (Dl) 70.7 ebony body (e) a. If ies heterozygous in cis position for javelin and ebony are mated among themselves, what phenotypic ratio do you expect in the progeny b. A true-breeding thread, ebony y is crossed with a true-breeding Delta y. An F1 female is testcrossed to a thread, ebony male. Predict the expected progeny and their frequencies for this cross. Assume no interference. c. Repeat b, but assume a coef cient of coincidence of 0.4. 13. Suppose that you have determined the order of three genes to be a, c, b, and that by doing two-point crosses you have determined map distances as a c 10 and c b 5. If interference is 1.5, and the three-point cross is ACB acb acb acb
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a. Are the two genes linked How do you know b. In the heterozygous females, were Trembling and Rex in cis or trans position Explain. c. Calculate the percent recombination between the two genes. 10. In corn, a trihybrid Tunicate (T ), Glossy (G), Liguled (L) plant was crossed with a nontunicate, nonglossy, liguleless plant, producing the following offspring:
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Tunicate, liguleless, Glossy Tunicate, liguleless, nonglossy Tunicate, Liguled, Glossy Tunicate, Liguled, nonglossy nontunicate, Liguled, Glossy nontunicate, Liguled, nonglossy nontunicate, liguleless, Glossy nontunicate, liguleless, nonglossy 58 15 55 13 16 53 14 59
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what frequency of double crossovers do you expect
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a. Determine which genes are linked. b. Determine the genotype of the heterozygote; be sure to indicate which alleles are on which chromosome. c. Calculate the map distances between the linked genes. 11. In Drosophila, kidney-shaped eye (k), cardinal eye (cd ), and ebony body (e) are three recessive genes. If homozygous kidney, cardinal females are crossed with homozygous ebony males, the F1 offspring are all wild-type. If heterozygous F1 females are mated with kidney, cardinal, ebony males, the following 2,000 progeny appear:
14. Given the following cross in Neurospora: ab a b , construct results showing that crossing over occurs in two of the four chromatids of a tetrad at meiosis. What would the results be if crossing over occurred during interphase before each chromosome became two chromatids if each crossover event involved three or four chromatids 15. A strain of yeast requiring both tyrosine (tyr ) and arginine (arg ) is crossed to the wild-type. After meiosis, the following ten asci are dissected. Classify each ascus as to segregational type (PD, NPD, TT). What is the linkage relationship between these two loci
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